In Vivo Differentiation of Therapeutic Insulin-Producing Cells from Bone Marrow Cells via Extracellular Vesicle-Mimetic Nanovesicles

摘要: The current diabetes mellitus pandemic constitutes an important global health problem. Reductions in the mass and function of β-cells contribute to most pathophysiology underlying diabetes. Thus, physiological control blood glucose levels can be adequately restored by replacing functioning β-cell mass. Sources functional islets for transplantation are limited, resulting great interest development alternate sources, recent progress regarding cell fate change via utilization extracellular vesicles, also known as exosomes microvesicles, is notable. this study investigated therapeutic capacity vesicle-mimetic nanovesicles (NVs) derived from a murine pancreatic line. To differentiate insulin-producing cells effectively, three-dimensional vivo microenvironment was constructed which NVs were applied subcutaneous Matrigel platforms containing bone marrow (BM) diabetic immunocompromised mice. Long-term achieved over 60 days, differentiation donor BM into platforms, composed islet-like clusters with extensive capillary networks, confirmed along expression key markers. resectioning caused rebound source β-cells. efficient attained through use NVs, maintained levels.