Cytokine Gene Expression in Peripheral Blood Mononuclear Cells and Tissues of Cattle Infected with Mycobacterium avium subsp. paratuberculosis: Evidence for an Inherent Proinflammatory Gene Expression Pattern

摘要: In cattle and other ruminants, infection with the intracellular pathogen Mycobacterium avium subsp. paratuberculosis results in a granulomatous enteritis (Johne's disease) that is often fatal. The key features of host immunity to M. include an appropriate early proinflammatory cytotoxic response (Th1-like) eventually gives way predominant antibody-based (Th2-like). Clinical disease symptoms appear subsequent waning Th1-like immune response. Understanding why this shift occurs underlying molecular mechanisms involved critical future control measures diagnosis. Previous studies have suggested may suppress gene expression peripheral blood mononuclear cells (PBMCs) from infected cows, despite continued inflammatory reaction at sites infection. present study, we tested hypothesis exposure suppresses pattern PBMCs cows. To do this, examined genes encoding interleukin-1α (IL-1α), IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p35, IL-16, IL-18, as well gamma interferon (IFN-γ), transforming growth factor β (TGF-β), tumor necrosis alpha (TNF-α), PBMCs, intestinal lesions, mesenteric lymph nodes naturally paratuberculosis. Cytokine these tissues was compared similar uninfected cattle. Our comprehensive demonstrate for most cytokine genes, including IFN-γ, TGF-β, TNF-α, IL-1α, differential did not require stimulation fact, tended reduce observed cows IL-6. Only IL-10 consistently enhanced by subclinically ileal paratuberculosis-infected cattle, IL-8 greater than comparable while IL-16 lower tissues. Mesenteric draining expressed higher levels mRNA expressed. contrast, TGF-β were Taken together, our suggest or capable limiting responses develop likely place development expansion cell populations