Characterization ofcis-Acting Elements of the Gene forMacrophage-stimulating Proteinfrom the Human
作者： Atsuhisa Ueda , Teizo Yoshimura
关键词: Molecular biology 、 Messenger RNA 、 Transcription (biology) 、 Electrophoretic mobility shift assay 、 Promoter 、 Base pair 、 Biology 、 CAAT box 、 Gene 、 Cell type 、 Cell biology 、 Biochemistry
摘要: To analyze the promotor region of human macrophage-stimulating protein (MSP) gene, 5′-flanking this gene was cloned. The major initiation site determined at T located 49 base pairs upstream translation by primer extention with mRNA from HepG2 and Hep3B cells. There no TATA sequence in region. Transient transfection assay 5′-deletion constructs showed that transcription regulated positive negative regulatory elements (PRE NRE). PRE (−34 to +2) essential for maximal NRE (−141 −34) appeared be responsible tissue-specific expression gene. contained CCAAT a mutation CTGAT resulted significant loss transcriptional activity. Electrophoretic mobility shift suggested two different proteins bound (MSP-PRE-binding protein-1 (MSP-PREB1) 2). MSP-PREB1 2 were detected various cell types, involved these bindings. These findings indicate are regulators. Further characterization also revealed MSP-PREB2 identical binding factor, known as NF-Y.
elsevier.com
highwire.org参考文章(49)
Muscatelli F, Breathnach R, Mattei Mg, Ronsin C, A novel putative receptor protein tyrosine kinase of the met family Oncogene. ,vol. 8, pp. 1195- 1202 ,(1993)
R. Mantovani, U. Pessara, F. Tronche, X.Y. Li, A.M. Knapp, J.L. Pasquali, C. Benoist, D. Mathis, Monoclonal antibodies to NF-Y define its function in MHC class II and albumin gene transcription. The EMBO Journal. ,vol. 11, pp. 3315- 3322 ,(1992) , 10.1002/J.1460-2075.1992.TB05410.X
A Iwama, K Okano, T Sudo, Y Matsuda, T Suda, Molecular cloning of a novel receptor tyrosine kinase gene, STK, derived from enriched hematopoietic stem cells. Blood. ,vol. 83, pp. 3160- 3169 ,(1994) , 10.1182/BLOOD.V83.11.3160.3160
M.H. Wang, S.L. Gonias, A. Skeel, B.B. Wolf, T. Yoshimura, E.J. Leonard, Proteolytic activation of single-chain precursor macrophage-stimulating protein by nerve growth factor-gamma and epidermal growth factor-binding protein, members of the kallikrein family. Journal of Biological Chemistry. ,vol. 269, pp. 13806- 13810 ,(1994) , 10.1016/S0021-9258(17)36719-4
T Vuorio, S N Maity, B de Crombrugghe, Purification and molecular cloning of the "A" chain of a rat heteromeric CCAAT-binding protein. Sequence identity with the yeast HAP3 transcription factor. Journal of Biological Chemistry. ,vol. 265, pp. 22480- 22486 ,(1990) , 10.1016/S0021-9258(18)45730-4
T. Yoshimura, N. Yuhki, M.H. Wang, A. Skeel, E.J. Leonard, Cloning, sequencing, and expression of human macrophage stimulating protein (MSP, MST1) confirms MSP as a member of the family of kringle proteins and locates the MSP gene on chromosome 3. Journal of Biological Chemistry. ,vol. 268, pp. 15461- 15468 ,(1993) , 10.1016/S0021-9258(18)82279-7
H Zorbas, T Rein, A Krause, K Hoffmann, E.L. Winnacker, Nuclear factor I (NF I) binds to an NF I-type site but not to the CCAAT site in the human alpha-globin gene promoter. Journal of Biological Chemistry. ,vol. 267, pp. 8478- 8484 ,(1992) , 10.1016/S0021-9258(18)42470-2
G. Gaudino, A. Follenzi, L. Naldini, C. Collesi, M. Santoro, K.A. Gallo, P.J. Godowski, P.M. Comoglio, RON is a heterodimeric tyrosine kinase receptor activated by the HGF homologue MSP. The EMBO Journal. ,vol. 13, pp. 3524- 3532 ,(1994) , 10.1002/J.1460-2075.1994.TB06659.X
M.H. Wang, T. Yoshimura, A. Skeel, E.J. Leonard, Proteolytic conversion of single chain precursor macrophage-stimulating protein to a biologically active heterodimer by contact enzymes of the coagulation cascade. Journal of Biological Chemistry. ,vol. 269, pp. 3436- 3440 ,(1994) , 10.1016/S0021-9258(17)41881-3
A Hatamochi, P T Golumbek, E Van Schaftingen, B de Crombrugghe, A CCAAT DNA binding factor consisting of two different components that are both required for DNA binding. Journal of Biological Chemistry. ,vol. 263, pp. 5940- 5947 ,(1988) , 10.1016/S0021-9258(18)60657-X