Enzymological Studies of Rat Luteinized Ovaries in Relation to Acute Reduction of Aromatizable Androgen Formation and Stimulated Production of Progestins

摘要: Female rats received an injection of 10 IU PMS gonadotropin (PMSG) sc at 21 days age. One group the animals was then injected with 30 hCG 23 age (PMSG-hCG treatment), and other not given (PMSG treatment). All were sacrificed 24 The ovarian weight increased by PMSG treatment further additional hCG, whereas uterine but decreased hCG. supernatant fluid obtained centrifugation 10,000 × g homogenates ovaries incubated 14C-labeled steroids in presence required cofactor to measure steroid-metabolizing enzyme activities. After PMSG-hCG treatment, activities 17α-hydroxylase C-17-C-20 lyase per mg protein or pair markedly compared those PMSG-treated group, aromatase 20α-hydroxysteroid dehydrogenase enhanced significantly. activity Δ5-3β-hydroxysteroid coupled...