Contributions from extracellular sources of adenosine to the ethanol toxicity in zebrafish larvae.

摘要: The effects of ethanol exposure on extracellular adenosine sources in zebrafish were evaluated. In the acute treatment, embryos exposed to 2% day 1 post-fertilization (dpf). chronic was continued for 2h/day up 6 dpf. Ecto-5'-nucleotidase activity assessed by colorimetric method and gene expression determined RT-qPCR 7 dpf zebrafish. Body length, ocular distance surface area eyes registered animals acutely pretreated with AOPCP (5-500 nM), an ecto-5'-nucleotidase inhibitor, or dipyridamole (10-100 μM), a blocker nucleoside transport. Both exposures promoted increased activity, impaired locomotion morphology. not affected. mild prevention morphological defects caused while worsened these defects. Early altered adenosinergic tonus, especially through transporters, contributing produced