Influence of Test Conditions on Antifungal Time-Kill Curve Results: Proposal for Standardized Methods

摘要: This study was designed to examine the effects of antifungal carryover, agitation, and starting inoculum on results time-kill tests conducted with various Candida species. Two isolates each albicans, tropicalis, glabrata were utilized. Test agents included fluconazole, amphotericin B, LY303366. Time-kill in RPMI 1640 medium buffered morpholinepropanesulfonic acid (MOPS) a pH 7.0 incubated at 35°C. Prior testing, existence carryover evaluated concentrations ranging from 1× 16× MIC by four plating methods: direct 10, 30, 100 μl test suspension filtration 30 through 0.45-μm-pore-size filter. curves performed isolate drug equal 2× MIC, using approximately 105 CFU/ml, or without agitation. Last, experiments over three ranges inocula: 5 × 102 1 104, >1 104 106, 106 108 CFU/ml. Significant (>25% reduction CFU/milliliter control value) observed B fluconazole; however, eliminated filtration. Agitation did not appreciably affect results. The significantly activity fluconazole B; LY303366 may be influenced inoculum. Before curve methods are routinely employed investigators, methodology should scrutinized standardized procedures developed.