Oxidation of cholesterol moiety of low density lipoprotein in the presence of human endothelial cells or Cu+2 ions: Identification of major products and their effects
作者： S. Bhadra , M.A.Q. Arshad , Z. Rymaszewski , E. Norman , R. Wherley
DOI: 10.1016/0006-291X(91)90942-Z
关键词: Biological activity 、 Reverse cholesterol transport 、 Umbilical vein 、 Endothelial stem cell 、 Stimulation 、 In vitro 、 Chemistry 、 Low-density lipoprotein 、 Cholesterol 、 Biochemistry
摘要: Abstract Oxidation of lipoproteins is believed to play a key role in atherogenesis. In this study, low density (LDL) was subjected oxidation the presence either human umbilical vein endothelial cells or with Cu+2 ions and major oxides formed were identified. While cholesterol-α-epoxide (C-αEP) product cholesterol peroxidation cells, cholest-3,5-dien-7-one (CD) predominated ion. Both steroids identified by gas chromatography/mass spectrometry. HDL resistant oxidation. When tested on skin fibroblasts culture C-αEP (10 μg/ml) caused marked stimulation 14C-oleate incorporation into esters, while CD stimulated esterification only mildly. These studies show that a) LDL moiety b) it causes cells. may increasing noted
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