Distribution of NADPH-diaphorase and expression of nNOS, N-methyl-D-aspartate receptor (NMDAR1) and non-NMDA glutamate receptor (GlutR2) genes in the neurons of the hippocampus after domoic acid-induced lesions in adult rats.

作者: C. Ananth , S. Thameem Dheen , P. Gopalakrishnakone , C. Kaur

DOI: 10.1002/HIPO.10060

关键词: HippocampusDentate gyrusNissl bodyGlutamate receptorHippocampal formationChemistryNeurotoxicityNitric oxideNeuroscienceIn situ hybridization

摘要: Neuronal degeneration followed by detection of nitric oxide (NO)-producing neurons the hippocampus was investigated at 4 h, 16 24 2 days, 5 and 14 days after administration domoic acid (DA), in present study. Histopathological analysis (Nissl staining) displayed dark-stained degenerating h to DA administration, with most severe 5–14 days. NADPH-d-positive were observed different subfields control rats treated 4–24 h. Complete loss CA1 CA3 also hilus dentate gyrus (DG) DA. In contrast, neuronal synthase (nNOS)-immunoreactive cells absent from hippocampal DA-treated animals but administration. N-methyl-D-aspartate receptor (NMDAR1) immunoreactivity increased double immunofluorescence demonstrated its coexpression induced nNOS expression. No significant change could be non-NMDA (GlutR2) as compared controls, while occasional immunoreactive colocalized Reverse transcription-polymerase chain reaction showed upregulated expression downregulated NMDAR1 Although mRNA rapidly situ hybridization revealed complete region The study has shown that NADPH-d express differentially DA-induced neurotoxicity. It is speculated induction glutamate genes response neurotoxicity have contributed degeneration. Hippocampus 2003;13:260–272. © 2003 Wiley-Liss, Inc.

参考文章(42)
Yang Wang, Philip A. Marsden, Nitric oxide synthases: gene structure and regulation. Advances in pharmacology (San Diego). ,vol. 34, pp. 71- 90 ,(1995) , 10.1016/S1054-3589(08)61081-9
Yukitoshi Izumi, Ann M. Benz, David B. Clifford, Charles F. Zorumski, Nitric oxide inhibitors attenuate excitotoxicity in rat hippocampal slices Neuroscience Letters. ,vol. 135, pp. 227- 230 ,(1992) , 10.1016/0304-3940(92)90442-A
JB Schulz, RT Matthews, BG Jenkins, RJ Ferrante, D Siwek, DR Henshaw, PB Cipolloni, P Mecocci, NW Kowall, BR Rosen, Blockade of neuronal nitric oxide synthase protects against excitotoxicity in vivo. The Journal of Neuroscience. ,vol. 15, pp. 8419- 8429 ,(1995) , 10.1523/JNEUROSCI.15-12-08419.1995
Xiaolin Deng, Jean Lud Cadet, Methamphetamine administration causes overexpression of nNOS in the mouse striatum. Brain Research. ,vol. 851, pp. 254- 257 ,(1999) , 10.1016/S0006-8993(99)02087-9
E. Bonfoco, D. Krainc, M. Ankarcrona, P. Nicotera, S. A. Lipton, Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 7162- 7166 ,(1995) , 10.1073/PNAS.92.16.7162
Carl Nathan, Qiao-wen Xie, Nitric oxide synthases: Roles, tolls, and controls Cell. ,vol. 78, pp. 915- 918 ,(1994) , 10.1016/0092-8674(94)90266-6
E Talavera, G Martınez-Lorenzana, G Corkidi, M Léon-Olea, M Condes-Lara, None, NADPH-diaphorase-stained neurons after experimental epilepsy in rats. Nitric Oxide. ,vol. 1, pp. 484- 493 ,(1997) , 10.1006/NIOX.1997.0158
Gregory R. Stewart, Charles F. Zorumski, Madelon T. Price, John W. Olney, Domoic acid: A dementia-inducing excitotoxic food poison with kainic acid receptor specificity Experimental Neurology. ,vol. 110, pp. 127- 138 ,(1990) , 10.1016/0014-4886(90)90057-Y