Decreased survival in EGFR gene amplified vulvar carcinoma

摘要: Objective. We undertook an extensive molecular characterization of the epidermal growth factor receptor (EGFR) gene in vulvar squamous cell carcinomas to investigate EGFR mutation and/or genomic amplification and its association with protein expression, high-risk human papillomavirus (HPV) status clinical outcome. Methods. A cohort 51 cancer patients distributed across all FIGO stages was selected for immunohistochemistry (IHC) fluorescence situ hybridization. expression were correlated HPV status, mutational prognostic variables. Fisher's exact tests, Kaplan–Meier survival estimates a Cox proportional-hazards model utilized. Results. chromosome 7 high polysomy observed 12% 6% cases, respectively. IHC malignant tissue 3+ staining demonstrated 100% sensitivity 79% specificity detect amplification, yielding 39% positive predictive value. Decreased (pb0.025) associated more statistically robust 3.3 hazard ratio (pb0.005) that controlled age at diagnosis, stage lymph node metastasis. Univariate analysis confirmed absence (pb0.001). Common activating mutations not identified. Conclusion. subset carcinoma identified HPV-independent poor prognosis. Given response targeted therapy other tumor types, these may be candidates therapeutic strategies target pathway.