Oncogenic role of miR-17-92 cluster in anaplastic thyroid cancer cells

摘要: Micro RNAs (miRNAs) are non-coding small and constitute a novel class of negative gene regulators that found in both plants animals. Several miRNAs play crucial roles cancer cell growth. To identify specifically deregulated anaplastic thyroid (ATC) cells, we performed comprehensive analysis miRNA expressions ARO cells primary thyrocytes using microarrays. MiRNAs miR-17-92 cluster were overexpressed cells. We confirmed the overexpression those by Northern blot FRO In 3 6 clinical ATC samples, miR-17-3p miR-17-5p robustly lesions compared to adjacent normal tissue. investigate functional role these transfected with inhibitors, antisense oligonucleotides containing locked nucleic acids. Suppression caused complete growth arrest, presumably due caspase activation resulting apoptosis. MiR-17-5p or miR-19a inhibitor also induced strong reduction, but only led cellular senescence. On other hand, miR-18a moderately attenuated Thus, have clarified differences among members conclusion, findings suggest plays an important certain types ATCs could be target for treatment. (Cancer Sci 2008; 99: 1147–1154)