Novel CMS lines in pigeonpea [Cajanus cajan (L.) Millspaugh] derived from cytoplasmic substitutions, and their effective restoration and deployment in hybrid breeding

摘要: Abstract The availability of stable cytoplasmic male sterile (CMS or A) lines coupled with a robust restoration system (R lines) is an essential prerequisite for efficient hybrid breeding. CMS-enabled technology holds immense potential to enhance the long-stagnant productivity pigeonpea. In present investigation, substitutions were made in nuclear backgrounds early-maturing pigeonpea varieties lines. Three new CMS (ICPL 88039A, Pusa 992A, and DPP 3-2A) resulted from genetic crosses involving donors A2 (GT 288A) A4 (ICPA 2089) categories. addition visual inspection anthers, pollen-staining techniques scanning electron microscopy (SEM) analysis used confirm pollen sterility. Further, given relevance plant mitochondrial genome manifestation, 25 mitochondrion-specific DNA markers assayed on these newly developed A isogenic maintainer (B) polymorphism between 992A 992B as revealed by nad7a_del marker confirmed successful combination sterilizing cytoplasm (A4) nonrestoring background (Pusa 992). Such cytoplasm-specific are required A2-CMS well. assess ability, restorers crossed lines, consequence, promising A × R combinations exhibiting 100% fertility could be identified. parallel, we also analyzed inheritance patterns underlying using ICPL 88039A-derived F2 BC1F1 populations, established monogenic dominant model explain phenomenon restoration. summary, report development describe their effective deployment breeding