Development of a headspace solid-phase microextraction method combined with gas chromatography mass spectrometry for the determination of phthalate esters in cow milk

摘要: Abstract Phthalate esters released from plasticized polyvinyl chloride (PVC) tubing into raw cow milk during milking at dairy farms could be a potential source of contamination by phthalate in products. A method was developed for the determination these samples using headspace solid-phase microextraction (HS-SPME) technique. The were mixed with sodium and extracted 60 min 90 °C. phthalates collected on SPME fibre then desorbed GC injection port followed GC-MS analysis single ion monitoring (SIM) mode. extraction efficiency dependent fat content sample. Since each sample different, combined standard addition internal used quantification samples. recoveries two spiking levels over 90% except dimethyl phthalate. detection limit di-(2-ethylhexyl) (DEHP) 0.31 to 3.3 ng g −1 containing up 10.8% fat. This sufficiently sensitive detect phthalate, dibutyl (DBP) diethyl (DEP) groups (cow without PVC tubing). While similar found both types DBP DEP, level DEHP much higher (215.36 ng g ) than once (16.04 ng g ), indicating leaching milk.