Membrane cut-off does not influence results regarding the measurement of small molecules - a comparative study between 20- and 100-kDa catheters in hepatic microdialysis.

摘要: Summary Background Microdialysis is a method used to monitor hepatic tissue metabolism. Membranes with molecular cut-off of 20 kilodalton (kDa) are currently measure the small metabolites glucose, glycerol, lactate and pyruvate. Using membranes higher such as 100 kDa allows possibility measuring larger molecules but may affect results regarding molecules. The aim was compare microdialysis catheters 20 in measurement pig liver model. Methods Four were inserted into each experiment (n = 6). Two 20 kDa perfused Ringer acetate, two 100 kDa: one acetate hydroxyethyl starch (Voluven) at flow rate 0·3 μl min−1. Dialysate samples collected 40-min intervals analysed for pyruvate. Results Compared other catheters, 100-kDa tended dialysate concentrations molecules, difference reaching statistical significance case Concentrations measured by Voluven were, however, comparable 20-kDa catheters. Conclusions Microdialysis membrane can be equally monitoring pyruvate, lactate/pyruvate ratio if high osmotic solution perfuse catheters.