作者: Guillem Jg , Weinstein Ib , Jiang W , Kahn Sm , Lu Sh
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摘要: We have developed a rapid, nonradioactive large scale method for the detection of ras oncogenes in human tumors. DNA is amplified by polymerase chain reaction (PCR), and then digested with specific restriction enzymes to detect either endogenous or primer-mediated Restriction Fragment Length Polymorphisms (RFLPs). report here that three 15 colon tumors tested contain K-ras codon 12 aspartic acid mutations one, along HCT 116 carcinoma cell line, contains 13 mutation. On other hand, we did not H- mutation 25 esophageal 27 gastric cardia isolated from patients Lin-xing County, China. By incorporating nucleotide substitutions PCR primers, this can be applied towards non-radioactive screening virtually any genetic disease caused known point mutations.