Loop-mediated isothermal amplification of single pollen grains.
作者： Ali Bektaş , Ignacio Chapela
DOI: 10.1111/JIPB.12191
关键词:
摘要: The polymerase chain reaction (PCR) has been a reliable and fruitful method for many applications in ecology. Nevertheless, unavoidable technical instrumental requirements of PCR have limited its widespread application field situations. recent development isothermal DNA amplification methods provides an alternative to PCR, which circumvents key limitations direct the field. Being able analyze pollen cloud ecosystem would provide very useful ecological information, yet require field-enabled, high-throughput this potential be realized. Here, we demonstrate applicability loop-mediated (LAMP), technique, used analysis. We that LAMP can identify species from cloud, it amplify successfully with sensitivity down single grains, thus opening possibility field-based,
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