Oral Delivery of Double-Stranded RNA in Larvae of the Yellow Fever Mosquito, Aedes aegypti: Implications for Pest Mosquito Control

摘要: RNA interference has already proven itself to be a highly versatile molecular biology tool for understanding gene function in limited number of insect species, but its widespread use other species will dependent on the development easier methods double-stranded (dsRNA) delivery. This study demonstrates that can induced mosquito Aedes aegypti L. (Diptera: Culicidae) simply by soaking larvae solution dsRNA two hours. The mRNA transcripts β-tubulin, chitin synthase-1 and -2, heat shock protein 83 were reduced between 30 50% three days post-dsRNA treatment. was mixed with visible dye identify those individuals fed dsRNA, based an absence contained no within their guts, primary route entry is likely through gut epithelium. systemic insects, inducing measurable knock down expression tissues beyond gut. Silencing β-tubulin genes resulted growth and/or mortality larvae, demonstrating utility as potential larvicide. synthase-2 did not induce silencing only insects if they subsequently subjected stress. Drosophila melanogaster Meigen Drosophilidae) also soaked designed specifically target either own gene, or A. aegypti, significant seen treated targeting which suggests pesticides could species-limited.