Mouse Vein Graft Hemodynamic Manipulations to Enhance Experimental Utility

摘要: Mouse models serve as a tool to study vein graft failure. However, in wild-type mice, there is limited intimal hyperplasia, hampering efforts identify anti–intimal hyperplasia therapies. Furthermore, wall remodeling has not been well quantified mice. We hypothesized that simple hemodynamic manipulations can reproducibly augment and end points mouse grafts, thereby enhancing their experimental utility. inferior vena cava–to-carotid interposition isografts were completed using an anastomotic cuff technique. Three flow restriction executed by ligating outflow carotid branches, creating common stenosis, constructing midgraft stenosis. Flowmetry ultrasonography used perioperatively at day 28. All ligation strategies decreased the rate shear stress. Morphometry showed thickness increased 26% via branch 80% Despite similar mean rates stresses among three manipulations, waveform amplitudes lowest with The disordered of stenosis yielded poststenotic dilatation. creation generates clinically relevant (poor runoff) low stress offers technically flexible method for response. Midgraft exhibits positive remodeling. These reproducible approaches offer novel increasing utility models.