Procedure for human saphenous veins ex vivo perfusion and external reinforcement.

摘要: The mainstay of contemporary therapies for extensive occlusive arterial disease is venous bypass graft. However, its durability threatened by intimal hyperplasia (IH) that eventually leads to vessel occlusion and graft failure. Mechanical forces, particularly low shear stress high wall tension, are thought initiate sustain these cellular molecular changes, but their exact contribution remains be unraveled. To selectively evaluate the role pressure on biology IH, an ex vivo perfusion system (EVPS) was created perfuse segments human saphenous veins under regimen (high pressure). Further technical innovations allowed simultaneous two from same vein, one reinforced with external mesh. Veins were harvested using a no-touch technique immediately transferred laboratory assembly in EVPS. One segment freshly isolated vein not perfused (control, day 0). others up 7 days, being completely sheltered 4 mm (diameter) pressure, flow velocity, pulse rate continuously monitored adjusted mimic hemodynamic conditions prevailing femoral artery. Upon completion perfusion, dismounted used histological analysis. Under conditions, (arterial, mean = 100 Hg) sufficient generate IH remodeling veins. These alterations reduced presence polyester