Organotypic culture of normal, dysplastic and squamous cell carcinoma-derived oral cell lines reveals loss of spatial regulation of CD44 and p75NTR in malignancy

摘要: Oral squamous cell carcinomas (OSCC) often arise from dysplastic lesions. The role of cancer stem cells in tumour initiation is widely accepted, yet the potential existence pre-cancerous tissue has received little attention. Cell lines oral diseases ranging severity dysplasia to malignancy provide opportunity investigate involvement malignant progression dysplasia. Stem are functionally defined by their ability generate hierarchical structures consortium with spatial regulation. Organotypic cultures readily display hierarchy vitro; hence, this study, we compared expression cell-associated markers dermis-based organotypic epithelial normal (OKF6-TERT2), mild (DOK), severe (POE-9n) and OSCC (PE/CA P J15). Expression CD44, p75NTR, CD24 ALDH was studied monolayers flow cytometry immunohistochemistry. Spatial regulation CD44 p75NTR evident for (OKF6-TERT2) (DOK POE-9n) but lacking PJ15)-derived cells. not evident. All monolayer exhibited antigens activity (ALDEFLUOR® assay), a trend towards loss population heterogeneity that mirrored disease severity. In monolayer, increased FOXA1 decreased FOXA2 correlated severity, OCT3/4, Sox2 NANOG did not. We conclude give mechanisms underlie seen OSCC-derived