Characterization of the expression of MHC proteins in human embryonic stem cells
作者： M. Drukker , G. Katz , A. Urbach , M. Schuldiner , G. Markel
关键词:
摘要: Human embryonic stem (ES) cells are pluripotent that may be used in transplantation medicine. These can induced to differentiate into from the three germ layers both vivo and vitro. To determine whether human ES might rejected after transplantation, we examined cell surface expression of MHC proteins these cells. Our results show very low levels class I (MHC-I) on moderately increase vitro or differentiation. A dramatic induction MHC-I was observed when were treated with IFN-γ but not IFN-α -β. However, all IFNs differentiated MHC-II HLA-G expressed undifferentiated Ligands for natural killer receptors either absent their derivatives. In accordance, cytotoxic assays demonstrated only limited lysis initiate a histocompatibility databank cells, have isotyped several published lines leukocyte antigens. conclusion, our demonstrate express high thus transplantation.
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