An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration

摘要: Neurodegenerative diseases are frequently associated with a progressive loss of movement ability, reduced life span, and age-dependent neurodegeneration. To understand the mechanism these cellular events, their causal relationships each other, Drosophila melanogaster, its sophisticated genetic tools diverse behavioral features, used as disease models for assessing neurodegenerative phenotypes. Here we describe high-throughput method to analyze adult negative geotaxis behavior, an indication possible motor defects An automated machine is designed developed drive fly synchronization using initial electric impulse, later allowing recording behavior over course secs mins. Images from digitally recorded video then processed self-designed RflyDetection software statistical data manipulation. Different manually controlled assay based on single fly, this precise, fast, protocol allows acquisition more than hundreds flies simultaneously, providing efficient approach advance our understanding in underlying locomotor deficits