Quantitative Analysis of Climbing Defects in a Drosophila Model of Neurodegenerative Disorders.

摘要: Locomotive defects resulting from neurodegenerative disorders can be a late onset symptom of disease, following years subclinical degeneration, and thus current therapeutic treatment strategies are not curative. Through the use whole exome sequencing, an increasing number genes have been identified to play role in human locomotion. Despite identifying these genes, it is known how crucial normal locomotive functioning. Therefore, reliable assay, which utilizes model organisms elucidate order identify novel targets interest, needed more than ever. We designed sensitized version negative geotaxis assay that allows for detection milder earlier has ability evaluate over time. The performed glass graduated cylinder, sealed with wax barrier film. By threshold distance climbed 17.5 cm experiment duration 2 min we observed greater sensitivity detecting mild mobility dysfunctions. cost effective does require extensive training obtain highly reproducible results. This makes excellent technique screening candidate drugs Drosophila mutants locomotion defects.