Combined Plasma and Tissue Proteomic Study of Atherogenic Model Mouse: Approach To Elucidate Molecular Determinants in Atherosclerosis Development.

摘要: Atherogenic cardiovascular diseases are the major cause of mortality. Prevention and prediction incidents is important; however, biomarkers that directly reflect disease progression remain poorly investigated. To elucidate molecular determinants atherogenesis, proteomic approaches advantageous by using model animals for comparing changes occurring systematically (bloodstream) locally (lesion) in accordance with stages. We conducted differential mass spectrometric analysis between apolipoprotein E deficient (apoED) wild-type (wt) mice plasma arterial tissue both types obtained at four pathognomonic time points disease. A total 100 proteins 390 tissues were continuously detected throughout points; 29 identified common. Of those, 13 36 showed significant difference abundance apoED wt certain points. Importantly, we found quantitative variation patterns regarding did not always correspond tissues, resulting gaining insight into atherosclerotic plaque progression. These characteristic to be components inflammation, thrombus formation, vascular remodeling, suggesting drastic integrative alteration atherosclerosis development.