Hormonally inducible phosphorylation of a nuclear pool of ribosomal protein S6.

摘要: Thyrotropin releasing hormone (TRH) and epidermal growth factor induce the rapid phosphorylation of a basic, chromatin-associated protein present in GH4 rat pituitary cells also found primary hepatocyte culture. Cell fraction experiments indicate nucleolar localization for this protein. The has been purified to homogeneity from liver 23 amino acids its N-terminal sequence determined. There is complete homology between sequenced portion ribosomal S6. In vivo vitro phosphorylation, two-dimensional gel analysis tryptic phosphopeptide mapping support identity Our experimental data existence nuclear pool S6 whose inducible.