Identification of 40 S ribosomal protein S6 phosphorylation sites in Swiss mouse 3T3 fibroblasts stimulated with serum.
作者： H.R. Bandi , S. Ferrari , J. Krieg , H.E. Meyer , G. Thomas
DOI: 10.1016/S0021-9258(18)53642-5
关键词: Phosphorylated Peptide 、 Protein phosphorylation 、 Ribosomal protein s6 、 Biochemistry 、 Ribosomal protein 、 Peptide 、 Molecular biology 、 Cyanogen bromide 、 Cleavage (embryo) 、 Biology 、 Phosphorylation
摘要: All of the phosphorylation sites in 40 S ribosomal protein S6 derived from serum-stimulated Swiss mouse 3T3 cells are found within a small cyanogen bromide (CNBr) peptide carboxyl terminus, Lys218-Lys249. Further cleavage CNBr or intact with endoproteinase Lys-C (endo Lys-C) generated single phosphorylated peptide, implying that all resided either between Arg231 and Lys243 Lys249 if at was blocked by nearby residue. To discern these possibilities to identify sites, purified cleaved endo Lys-C, isolated sequenced following conversion serines S-ethylcysteine. The results show extends vivo Ser235, Ser240, Ser244, Ser247.
sci-hub.st 参考文章(28)
H.A. Lane, S.J. Morley, M. Dorée, S.C. Kozma, G. Thomas, Identification and early activation of a Xenopus laevis p70s6k following progesterone-induced meiotic maturation. The EMBO Journal. ,vol. 11, pp. 1743- 1749 ,(1992) , 10.1002/J.1460-2075.1992.TB05226.X
Raymond L Erikson, None, Structure, expression, and regulation of protein kinases involved in the phosphorylation of ribosomal protein S6. Journal of Biological Chemistry. ,vol. 266, pp. 6007- 6010 ,(1991) , 10.1016/S0021-9258(18)38072-4
Helmut E. Meyer, Edeltraut Hoffmann-Posorske, Ludwig M.G. Heilmeyer, Determination and location of phosphoserine in proteins and peptides by conversion to S-ethylcysteine. Methods in Enzymology. ,vol. 201, pp. 169- 185 ,(1991) , 10.1016/0076-6879(91)01016-U
Michel Siegmann, George Thomas, [34] Separation of multiple phosphorylated forms of 40 S ribosomal protein S6 by two-dimensional polyacrylamide gel electrophoresis Methods in Enzymology. ,vol. 146, pp. 362- 369 ,(1987) , 10.1016/S0076-6879(87)46037-0
R Franco, M G Rosenfeld, Hormonally inducible phosphorylation of a nuclear pool of ribosomal protein S6. Journal of Biological Chemistry. ,vol. 265, pp. 4321- 4325 ,(1990) , 10.1016/S0021-9258(19)39566-3
S D Brandon, R A Masaracchia, Multisite phosphorylation of a synthetic peptide derived from the carboxyl terminus of the ribosomal protein S6. Journal of Biological Chemistry. ,vol. 266, pp. 380- 385 ,(1991) , 10.1016/S0021-9258(18)52445-5
William J. Boyle, Peter van der Geer, Tony Hunter, Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates. Methods in Enzymology. ,vol. 201, pp. 110- 149 ,(1991) , 10.1016/0076-6879(91)01013-R
Helmut E. Meyer, Edeltraut Hoffmann-Posorske, Horst Korte, Ludwig M.G. Heilmeyer, Sequence analysis of phosphoserine-containing peptides. Modification for picomolar sensitivity. FEBS Letters. ,vol. 204, pp. 61- 66 ,(1986) , 10.1016/0014-5793(86)81388-6
S J Burkhard, J A Traugh, Changes in ribosome function by cAMP-dependent and cAMP-independent phosphorylation of ribosomal protein S6. Journal of Biological Chemistry. ,vol. 258, pp. 14003- 14008 ,(1983) , 10.1016/S0021-9258(17)44016-6
J Krieg, J Hofsteenge, G Thomas, Identification of the 40 S ribosomal protein S6 phosphorylation sites induced by cycloheximide. Journal of Biological Chemistry. ,vol. 263, pp. 11473- 11477 ,(1988) , 10.1016/S0021-9258(18)37981-X