Immunogenicity of axenic Entamoeba histolytica antigen and its fractions

摘要: Abstract Amoebic antigen prepared from axenic Entamoeba histolytica (NIH:200 strain) grown at 37 °C in TPS I medium was chromatographed on a Sephadex G-200 column. Three fractions (F-I, F-II, F-III) were obtained, the molecular weights of which ranged 650,000 to 1,450. Fractions concentrated by lyophilization and antisera against each fraction whole raised rabbits. Partially purified used various serological tests their reactivities compared with amoebic antigen. Main haemagglutinating precipitating activities confined Fraction I, had 14% total protein highest weight (650,000). 5 μg/ml contained this gave antibody titres comparable those obtained 40μg/ml protein. Other fractions, although they precipitin activity, lacked activity. The protective efficacies these are being investigated our laboratory.