Activated vascular endothelia regulate invasion of glioma cells through expression of fibronectin

摘要: Background Previous researches have indicated that glioma invasion may occur within a tumor-host microecology, and fibronectin be involved in as an important component of the extracellular matrix. However, how interaction between tumor cells vascular endothelial affects is poorly understood. The aim this study was to investigate effects on invasion, relationship fibronectin. Methods localization different brain astrocytoma tissues determined by immunohistochemistry. Then, were co-cultured Transwell co-culturing system. Fibronectin expression detected reverse transcriptase-polymerase chain reaction, immunocytochemistry, enzyme-linked immunosorbent assay. Additionally, influence cell vitro rapid test. Results In tissues, present cells, greater higher grade tumors than lower tumors. induced release from which turn stimulated migration. This effect inhibited blocking antibody. Conclusion Glioma induce epithelial express fibronectin, could promote invasion. Chin Med J 2010;123(13):1754-1761 lioma most common central nervous system it continues major therapeutic challenge. prognosis for malignant poor, with best 5-year survival being only 9.8% despite patients having undergone surgery, cranial radiation treatment, intensive chemotherapy. 1 One difficult problems treatment although gliomas are rarely found metastasize outside system, diffusive infiltration growth makes surgical removal impossible. Excessive proliferation surrounding key biological features phenotype glioma. 2 research has occurs microecology. 3 recruit vasculature stroma through production secretion stimulatory factors cytokines. 4 locally activated host microenvironment (including both cellular elements) modifies proliferative invasive behavior cells. 5,6 Host include immune inflammatory muscle myofibroblasts, etc. 3,7 We previously performed studies immunohistochemistry laminin (LN), (FN), P53, microecosystem (TIMES) human glioma, micrangium play role