Structure–activity relationships of the ATP cofactor in ligase-catalysed oligonucleotide polymerisations
DOI: 10.1039/C6OB02792J
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摘要: A T4 DNA ligase-catalysed oligonucleotide polymerisation process has been recently developed to enable the incorporation of multiple functional groups throughout a nucleic acid polymer. ligase requires ATP as cofactor catalyse phosphodiester bond formation during process. Herein, we describe structure–activity relationship within context ligase-catalyzed polymerisation. Using high-throughput sequencing, study not only influence modification on efficiency, but also fidelity and sequence bias
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