Anchorage and lymphocyte function II. Contact with non-cellular surfaces, cell density and T-cell activation.

摘要: Varying numbers of human blood lymphocytes were stimulated by concanavalin A (Con-A) in a constant medium volume. At 'low' cell densities DNA synthesis was proportional to the number cells cultures whereas at 'high' considerably lower than expected. In presence non-mitogenic microcarrier beads, (diameter 195 micrometer) which attached, or nearly also densities. The potentiating effect beads on lymphocyte stimulation particularly noteworthy individuals showing weak mitogen response. Another approach that yielded proportionality between and both use culture vessels with larger bottom area. Under such conditions did not augment synthesis. These results suggest availability non-cellular adhesive surface is major limiting factor density regulating control activation. Anchorage may modulate dependent 'growth mechanism' indirectly via an influence cell-cell interaction. An alternative less-likely interpretation contact surfaces directly gives rise regulatory responses accessory cells.