Permissive effect of substratum contact on DNA synthesis in concanavalin A-treated lymphocytes.

摘要: The effects of contact with a solid surface (in the form adhesive microcarrier beads (Cytodex)) on induction proliferation human blood lymphocytes after exposure to concanavalin A (Con A) were investigated. duration lag phase preceding DNA synthesis was identical in presence and absence beads. kinetic course initiation exponential but different rate constants both during first 4 days culture period. Cytodex exerted its effect by increasing proportion cells initiating response Con A. To elucidate whether responded dose-dependent manner, seeded at concentrations exposed for times. At 36 48 h start stimulation synthesizing increased cell number cultures. In contrast, portion markedly decreased high densities 60, 72 96 stimulation. beads, however, proportional cultures also h.