Fibronectin mediates cytokinesis and growth of rat follicular cells in serum-free medium

作者: Joseph Orly , Gordon Sato

DOI: 10.1016/0092-8674(79)90155-7

关键词:

摘要: In this study, the growth of rat follicular (RF-1) cells was severely depressed when were subcultured by trypsinization directly into serum-free medium supplemented with insulin, transferrin and hydrocortisone, which are required for these in vitro. Within 24 hr after plating, 50-65% became binucleated, indicating lack cytokinesis. However, addition human plasma fibronectin (8 microgram/ml) to eliminated cell binucleation enhanced considerably. Fibronectin had same effect RF-1 plated tissue culture dishes on been absorbed, inoculated fibronectin-free medium. Cell poor occurred trypsinization, EGTA treatment or detachment mitotic cells. Under some conditions, could be "rescued" if added soon inoculation, that needed mainly during a limited time attaching substratum. Our findings describe an adhesive activity circumvents serum preincubation usually They also indicate importance fibronectin-mediated adhesion cytokinesis processes

参考文章(51)
Lloyd A. Culp, Biochemical Determinants of Cell Adhesion Current topics in membranes and transport. ,vol. 11, pp. 327- 396 ,(1978) , 10.1016/S0070-2161(08)60752-2
M. Abercrombie, Joan E.M. Heaysman, Susan M. Pegrum, The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella. Experimental Cell Research. ,vol. 67, pp. 359- 367 ,(1971) , 10.1016/0014-4827(71)90420-4
F. Grinnell, Cell spreading factor. Occurrence and specificity of action Experimental Cell Research. ,vol. 102, pp. 51- 62 ,(1976) , 10.1016/0014-4827(76)90298-6
Frederick Grinnell, Donald G. Hays, Cell adhesion and spreading factor. Similarity to cold insoluble globulin in human serum Experimental Cell Research. ,vol. 115, pp. 221- 229 ,(1978) , 10.1016/0014-4827(78)90419-6
Jane Bottenstein, Izumi Hayashi, Sharon Hutchings, Hideo Masui, Jennie Mather, Don B. McClure, Sugayuki Ohasa, Angie Rizzino, Gordon Sato, Ginette Serrero, Richard Wolfe, Reen Wu, [6] The growth of cells in serum-free hormone-supplemented media Methods in Enzymology. ,vol. 58, pp. 94- 109 ,(1979) , 10.1016/S0076-6879(79)58127-0
R. O. Hynes, Alteration of cell-surface proteins by viral transformation and by proteolysis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 3170- 3174 ,(1973) , 10.1073/PNAS.70.11.3170
Magnus Höök, Kristofer Rubin, Åke Oldberg, Björn Öbrink, Antti Vaheri, Cold-insoluble globulin mediates the adhesion of rat liver cells to plastic petri dishes Biochemical and Biophysical Research Communications. ,vol. 79, pp. 726- 733 ,(1977) , 10.1016/0006-291X(77)91172-X
S. Lustig, N. S. Kosower, D. H. Pluznik, E. M. Kosower, Inhibition of cytokinesis in Friend leukemia cells by membrane mobility agents. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 2884- 2888 ,(1977) , 10.1073/PNAS.74.7.2884