Expression of rotavirus capsid protein VP6 in transgenic potato and its oral immunogenicity in mice.
作者： Jie Yu , William Langridge
关键词:
摘要: Murine rotavirus gene six encoding the 41 kDa group specific capsid structural protein VP6 was stably inserted into Solanum tuberosum genome by Agrobacterium tumefaciens mediated transformation. The molecular mass of plant synthesized determined immunoblot similar to size both purified virus monomeric peptides and partially assembled virus-like particles. amount in transgenic potato leaf tuber enzyme-linked immunosorbent assay be approximately 0.01% total soluble protein. Oral immunization CD-1 mice with transformed tissues containing generated measurable titers anti-VP6 serum IgG intestinal IgA antibodies. presence detectable humoral antibody responses against following mucosal provides an optimistic basis for development edible vaccines enteric viral pathogens.
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