Synthesis and characterization of CS/TPP nanoparticles for oral delivery of gene in fish
作者： S Vimal , G Taju , KS Nathiga Nambi , S Abdul Majeed , V Sarath Babu
DOI: 10.1016/J.AQUACULTURE.2012.06.012
关键词:
摘要: This work examines the potential use of chitosan tripolyphosphate (CS/TPP) nanoparticles for gene delivery in different tissues fish through oral route. The porin Vibrio anguillarum was used to construct a DNA vaccine using pcDNA 3.1, eukaryotic expression vector and named as pVAOMP. CS/TPP were synthesized by ionic gelation process these particles characterized. morphology particle size measurements studied field emission scanning electron microscopy (FE-SEM) FTIR (Fourier Transform Infrared Spectra). pVAOMP encapsulated mixing equal volume heated solutions. encapsulation efficiency found be 79.9% binding with nanoparticles. stability plasmid also determined after DNase I chitosanase. cytotoxicity evaluated MTT assay cell line. confirmed immunohistochemistry, ELISA real-time PCR analyses. results indicate that can easily delivered into feeding
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