Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using bovine (Bos taurus L.) X- and Y-painting probes

摘要: River buffalo, sheep, and goat spermatozoa were cross-hybridized using double color fluorescence in situ hybridization (FISH) with bovine Xcen- Y-chromosome painting probes, prepared by DOP-PCR of laser-microdissected-catapulted chromosomes, to investigate the possibility probes for sexing sperm other members family Bovidae. Before analysis, hybridized on metaphase chromosomes each species, as control. Frozen-thawed cattle, river decondensed suspension 5 mM DTT. Sperm samples obtained from three individuals species investigated, more than 1,000 scored animal. FISH analysis 12,000 revealed high level X- or Y-signals all indicating efficiency over 99%. Significant interspecific differences detected frequency aberrant (aneuploid diploid) between (0.393%) sheep (0.033%) (P < 0.01), cattle (0.096%) (P < 0.5), well buffalo (0.224%) (P < 0.5). There was no significant difference cattle. The present study demonstrated that it is possible use X-Y analyzing family, thus facilitating future studies incidence chromosome abnormalities sex predetermination embryos livestock industry. Mol. Reprod. Dev. 67: 108–115, 2004. © 2004 Wiley-Liss, Inc.