Determination of the Correlation Between Stallion’s Age and Number of Sex Chromosome Aberrations in Spermatozoa
作者： M Bugno-Poniewierska , D Kozub , K Pawlina , M Tischner , M Tischner
DOI: 10.1111/J.1439-0531.2010.01742.X
关键词: Biology 、 Chromosome aberration 、 Andrology 、 X chromosome 、 Y chromosome 、 Ploidy 、 Aneuploidy 、 Sperm 、 Semen 、 Trisomy
摘要: Contents The aim of this study was a cytogenetic analysis stallions semen to find sex chromosome aberrations and determine if there an association between stallion’s age aberration frequency for the chromosomes. Sperm samples were collected from 22 various 3 23 years. Multicolour FISH performed on each sample, using probes chromosomes EGFR gene, localized 4p12 in domestic horse. A total 26199 sperm cells analysed (from 1 070 532 per animal). Among cells, 50.318% with X chromosome, 48.543% Y 1.139% aberrant The was: nullisomy (0.466%), XY aneuploidy (0.454%), XX disomy (0.146%), YY (0.041%), diploidy (0.024%) trisomy XXY (0.008%). Additionally correlation animal significant positive XY, XX, YY, XXY, autosomal seen. Correlation stallion level negative. present demonstrated that technique is powerful method identify equine spermatozoa might be very helpful breeder during selection best stallion.
sci-hub.se 参考文章(22)
R H Martin, E Ko, E Spriggs, A W Rademaker, The relationship between paternal age, sex ratios, and aneuploidy frequencies in human sperm, as assessed by multicolor FISH American Journal of Human Genetics. ,vol. 57, pp. 1395- 1399 ,(1995)
J. Kobayashi, T. Kohsaka, H. Sasada, M. Umezu, E. Sato, Fluorescence in situ hybridization with Y chromosome-specific probe in decondensed bovine spermatozoa. Theriogenology. ,vol. 52, pp. 1043- 1054 ,(1999) , 10.1016/S0093-691X(99)00193-4
Tatsuo Kawarasaki, Tetsuya Kohsaka, Masaru Sone, Mitsutoshi Yoshida, Kimio Bamba, None, Detection of Y‐bearing porcine spermatozoa by in situ hybridization using digoxigenin‐labeled, porcine male‐specific DNA probe produced by polymerase chain reaction Molecular Reproduction and Development. ,vol. 40, pp. 455- 459 ,(1995) , 10.1002/MRD.1080400409
Darren K. Griffin, Michael A. Abruzzo, Elise A. Millie, Leon A. Sheean, Eleanor Feingold, Stephanie L. Sherman, Terry J. Hassold, Non-disjunction in human sperm : Evidence for an effect of increasing paternal age Human Molecular Genetics. ,vol. 4, pp. 2227- 2232 ,(1995) , 10.1093/HMG/4.12.2227
Dino Di Berardino, Miluse Vozdova, Svatava Kubickova, Halina Cernohorska, Giuseppe Coppola, Gianfranco Coppola, Giuseppe Enne, Jiri Rubes, Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using bovine (Bos taurus L.) X- and Y-painting probes Molecular Reproduction and Development. ,vol. 67, pp. 108- 115 ,(2004) , 10.1002/MRD.20016
Tatsuo Kawarasaki, Masaru Sone, Mitsutoshi Yoshida, Kimio Bamba, Rapid and simultaneous detection of chromosome Y- and 1-bearing porcine spermatozoa by fluorescence in situ hybridization Molecular Reproduction and Development. ,vol. 43, pp. 548- 553 ,(1996) , 10.1002/(SICI)1098-2795(199604)43:4<548::AID-MRD18>3.0.CO;2-V
Mohamed Hassanane, Andras Kovacs, Pascal Laurent, Kerstin Lindblad, Ingemar Gustavsson, Simultaneous detection of X- and Y-bearing bull spermatozoa by double colour fluorescence in situ hybridization Molecular Reproduction and Development. ,vol. 53, pp. 407- 412 ,(1999) , 10.1002/(SICI)1098-2795(199908)53:4<407::AID-MRD6>3.0.CO;2-O
J. Rubeš, M. Vozdová, S. Kubíčková, Aneuploidy in pig sperm: multicolor fluorescence in situ hybridization using probes for chromosomes 1, 10, and Y. Cytogenetic and Genome Research. ,vol. 85, pp. 200- 204 ,(1999) , 10.1159/000015293
Monika Bugno-Poniewierska, Zofia Jabłońska, Ewa Słota, Modification of equine sperm chromatin decondensation method to use fluorescence in situ hybridization (FISH). Folia Histochemica Et Cytobiologica. ,vol. 47, pp. 663- 666 ,(2010) , 10.2478/V10042-010-0006-2
B. Kinakin, A. Rademaker, R. Martin, Paternal age effect of YY aneuploidy in human sperm, as assessed by fluorescence in situ hybridization Cytogenetic and Genome Research. ,vol. 78, pp. 116- 119 ,(1997) , 10.1159/000134641