Fertility Assessment in Sorraia Stallions by Sperm-Fish and Fkbp6 Genotyping

摘要: The Sorraia, a critically endangered indigenous Iberian horse breed, is characterized by low genetic variability, high rate of inbreeding, bad sperm quality and subfertility. Here, we studied 11 phenotypically normal but subfertile Sorraia stallions karyotyping, sex chromosome sperm-FISH molecular analysis FKBP6 - susceptibility locus for impaired acrosome reaction (IAR). had concentration (>300 million cells/ml), the numbers progressively motile (21%) morphologically (28%) were invariably low. All 64,XY karyotype. majority (89%) haploid content, although 11% carried various aneuploidies. No correlation was found between percentage abnormalities morphology or stallion age. Direct sequencing exon 4 SNPs g.11040315G>A g.11040379C>A revealed that none genotype (A/A-A/A) IAR. Instead, all animals G/G-A/A testimony variability. findings ruled out chromosomal predisposition IAR as contributing factors However, fertility could be partly attributed to relatively higher aneuploidies in sperm.