Induction of proliferating cell nuclear antigen (PCNA)-immunoreactive cells in goldfish retina following intravitreal injection with 6-hydroxydopamine.

摘要: 1. The dopaminergic neurotoxin, 6-hydroxydopamine (6-OHDA), was injected intravitreally into the eyes of juvenile (5- to 6-cm) goldfish. 2. Proliferation rod neuroblasts caused by 6-OHDA (2µg in 2µl saline) detected retinal wholemounts immunofluorescence for proliferating cell nuclear antigen (PCNA) 3, 7, 14, 20, or 30 days after injection. 3. The dose sufficient cause permanent loss interplexiform and serotonergic amacrine cells eye but not contralateral control eye. 4. 6-OHDA increased density (mm−2) PCNA-ir theouter layer (ONL) 2.65 times initial 20–30 injection, it ONL contralateral, untreated eye, equally a delay ⩽7 with respect eye. 5. 6-OHDA also theinner (INL) >20 7 followed rapid decline almost levels 14 injection. 6. The sequence responses 6-OHDA, first scattered then clustered INL, suggests that from migrate INL compensate toxin-induced loss. 7. Double staining 5-bromodeoxyuridine (BrUdR; thymidine analogue) PCNA, carried out on intravitreal injection showed 77% all outer had been S phase during previous 24 hr. 8. Immunoreactivity PCNA found be valid marker which have entered within 1–2 before sampling shown especially convenient investigating distribution whole mounts. 9. In controls unilaterally saline plus 1% dimethyl sulfoxide (DMSO), differences densities precursor nuclei 2–30 vs. day 0 (uninjected) were statistically insignificant both uninjected (Negishiet al., 1991). Therefore local effect injecting due itself, mechanical damage nonspecific actions foreign substances. 10. The proliferative response could part impairment regulation mitogen(s) released damaged cells. The theuninjected however, is likely diffusible treated retina.