Single-stranded DNA binding protein Ssbp3 induces differentiation of mouse embryonic stem cells into trophoblast-like cells

摘要: Intrinsic factors and extrinsic signals which control unlimited self-renewal developmental pluripotency in embryonic stem cells (ESCs) have been extensively investigated. However, a much smaller number of involved extra-embryonic trophoblast differentiation from ESCs studied. In this study, we investigated the role single-stranded DNA binding protein, Ssbp3, for induction trophoblast-like mouse ESCs. Gain- loss-of-function experiments were carried out through overexpression or knockdown Ssbp3 under culture conditions. Expression levels lineage markers detected by real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analyses. The global gene expression profile Ssbp3-overexpressing was determined affymetrix microarray. Gene ontology pathway terms analyzed further validated qRT-PCR Western blotting. methylation status Elf5 promoter bisulfite sequencing. phenotype induced also evaluated teratoma formation early embryo injection assays. Forced upregulated lineage-associated genes, with cell being highest. contrast, depletion attenuated marker genes downregulation Oct4 treatment BMP4 bFGF Interestingly, profiling analysis indicated that did not significantly alter transcript pluripotency-associated transcription factors. Instead, promoted trophectoderm such as Cdx2 activated MAPK/Erk1/2 TGF-β pathways. Furthermore, reduced level generation teratomas internal hemorrhage, indicative presence cells. This study identifies regulator to differentiate into finding is helpful understand regulatory networks ESC lineages.