Expression of salt and urea transporters in rat kidney during cisplatin-induced polyuria

摘要: Expression of salt and urea transporters in rat kidney during cisplatin-induced polyuria. Background Cisplatin (CP) induced polyuria rats is associated with a reduction medullary hypertonicity, normally generated by the thick ascending limb (TAL) transporters, collecting duct (UT). To investigate molecular basis this abnormality, we determined protein abundance major UT isoforms CP-induced Methods Male Sprague-Dawley received either single injection CP (5 mg/kg, N = 6) or saline ( intraperitoneally five days before sacrifice. Urine, blood, kidneys were collected analyzed. Results CP-treated developed polyuric acute renal failure as assessed increased blood nitrogen (BUN), urine volume decreased osmolality. Western analysis homogenates revealed marked band density bumetanide-sensitive Na-K-2Cl cotransporter cortex (60% control values, P Conclusions Changes outer inner are unlikely to play role hypertonicity. However, qualitative changes proteins may affect their functionality thus have role.