Expression and function of angiopoietin-1 in breast cancer

摘要: Angiopoietin-1 (Ang1) has been shown to act as an angiogenic promoter in embryonic angiogenesis by promoting vascular branching, pericyte recruitment and endothelial survival. We have investigated the role of Ang1 tumour neovascularization under clinical conditions animal models. The expression breast cancer specimens was analysed using laser-capture microdissection reverse transcriptase-linked polymerase chain reaction (RT-PCR) on RNA isolated from samples. Despite many human cell lines, gene expressed only three 21 specimens, even though its receptor, Tie2, is abundant vasculature all these tumours. then overexpressed a line (MCF-7) own conjunction with FGF1, factor be able increase tumorigenicity MCF-7 cells. High concentrations were produced conditioned media transfected cells (range 156–820 ng ml–1). However, contrast physiological angiogenesis, overexpression did not enhance growth, but instead caused up 3-fold retardation growth (P = 0.003). © 2000 Cancer Research Campaign