Differential regulation and expression of hyaluronan synthases in human articular chondrocytes, synovial cells and osteosarcoma cells.

摘要: Recently three isoforms of hyaluronan synthase (HAS), the enzyme responsible for hyaluronate/hyaluronan (HA) biosynthesis, have been cloned, allowing us to study their expression pattern. Our objective was determine which HAS isoenzymes were expressed in human articular chondrocytes, synovial fibroblasts and osteosarcoma cells, whether could be modulated by growth factors (insulin-like factor-1, basic fibroblast factor transforming (TGF-beta1) cytokines [interleukin 1beta1 (IL-1beta)], changes rate HA synthesis cells correlated with mRNA levels one or more isoforms. All found cultured analysed this study, although relative proportions varied each cell type. HAS2 usually predominant whereas contained increased amounts HAS1. HAS3 always least abundant message. The rapidly growing almost exclusively usage uncultured cartilage tissues similar that message being species HAS1 synovium. stimulated factors, but extent response cell-type specific. Synovial responded particularly well IL-1beta, showed a unique synergistic when IL-1beta used combination TGF-beta1. This much reduced chondrocytes absent cells. Analysis indicated there often no correlation secretion following exposure factors. Although HAS-1 after TGF-beta1/IL-1beta, magnitude change far less than effect on synthesis. data thus suggest gene is tissue specific, regulation further cell-specific In addition, biosynthesis appears multi-faceted, control only aspect process.