Suppression of glioblastoma angiogenicity and tumorigenicity by inhibition of endogenous expression of vascular endothelial growth factor

摘要: Abstract The development of new capillary networks from the normal microvasculature host appears to be required for growth solid tumors. Tumor cells influence this process by producing both inhibitors and positive effectors angiogenesis. Among latter, vascular endothelial factor (VEGF) has assumed prime candidacy as a major physiological effector. Here, we have directly tested hypothesis in brain tumor, glioblastoma multiforme, one most highly vascularized human cancers. We introduced an antisense VEGF expression construct into found that (i) mRNA protein levels were markedly reduced, (ii) modified did not secrete sufficient factors so chemoattractive primary microvascular cells, (iii) able sustain tumor immunodeficient animals, (iv) density vivo blood vessel formation was reduced direct relation reduction secretion formation. Moreover, revertant recovered ability regained each these tumorigenic properties. These results suggest plays angiogenic role glioblastoma.