Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs.
作者： Li Yi , Shipeng Cheng , Hongli Xu , Jianke Wang , Yuening Cheng
DOI: 10.1016/J.JVIROMET.2011.11.011
关键词:
摘要: A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type vaccine strains canine distemper virus (CDV). pair primers (P1/P2) used to detect both CDV vaccines. Another (P3/P4) only strains. 335bp fragment amplified from genomic RNA 555bp specifically No amplification achieved uninfected cells, cells infected with parvovirus, coronavirus, or adenovirus. The RT-PCR detected effectively differentiated by two separate RT-PCRs. can be clinical epidemiological surveillance. phylogenetic analysis hemagglutinin gene local revealed that seven isolates all belonged Asia-1 lineage, were clustered closely one another at same location. These results suggested genotype is circulating currently in domestic dogs China.
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