Real-time reverse transcription polymerase chain reaction method for detection of Canine distemper virus modified live vaccine shedding for differentiation from infection with wild-type strains:

摘要: Canine distemper virus (CDV) remains a common cause of infectious disease in dogs, particularly high-density housing situations such as shelters. Vaccination all dogs against CDV is recommended at the time admission to animal shelters and many use modified live (MLV) vaccine. From diagnostic standpoint for with suspected infection, this problematic because highly sensitive real-time reverse transcription polymerase chain reaction (RT-PCR) tests are able detect MLV clinical samples. Real-time PCR can be used quantitate amount shedding differentiate vaccine strains from wild-type when high. However, differentiation by quantitation not possible vaccinated animals during acute low could mistaken level shedding. While there gel-based RT-PCR assays field based on sequence differences, sensitivity o...