FOXM1 Inhibition in Ovarian Cancer Tissue Cultures Affects Individual Treatment Susceptibility Ex Vivo.

摘要: Diagnosis in an advanced state is a major hallmark of ovarian cancer and recurrence after first line treatment common. With upcoming novel therapies, tumor markers that support patient stratification are urgently needed to prevent ineffective therapy. Therefore, the transcription factor FOXM1 promising target as it frequently overexpressed associated with poor prognosis. In this study, fresh tissue specimens 10 cancers were collected investigate cultures their ability predict individual susceptibility identify benefit inhibition. inhibition was induced by thiostrepton (3 µM). Carboplatin (0.2, 2 20 µM) olaparib (10 applied analyzed cell proliferation apoptosis immunofluorescence microscopy. Resistance mechanisms investigated determining gene expression its targets BRCA1/2 RAD51. Ovarian successfully maintained for up 14 days ex vivo, preserving morphological characteristics native specimen. Thiostrepton downregulated culture. Individual responses observed combined carboplatin or olaparib. Thus, we implemented complex culture model showed potential