An In Situ Hybridization Protocol for Detection and Identification of Planktonic Bacteria
作者： Frank Oliver Glöckner , Rudolf Amann , Albin Alfreider , Jakob Pernthaler , Roland Psenner
DOI: 10.1016/S0723-2020(96)80069-5
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摘要: Summary The fraction of planktonic bacteria in oligo- and mesotrophic lakes that can be classified by situ hybridization has been significantly increased with an optimized protocol. By the use white polycarbonate filters, CY3-monolabeled oligonucleotide probes, high quality fluorescence filter sets between 29 64% cells visualized 4',6-diamidino-2-phenylindole (DAPI) detected a bacterial probe or group-specific probes epifluorescence microscopy without further digital image enhancement.
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