Serratia marcescens is injurious to intestinal epithelial cells.

摘要: Diarrhea causes substantial morbidity and mortality in children low-income countries. Although numerous pathogens cause diarrhea, the etiology of many episodes remains unknown. Serratia marcescens is incriminated hospital-associated infections, HIV/AIDS associated diarrhea. We have recently found that spp. may be more commonly stools patients with diarrhea than asymptomatic control children. therefore investigated possible enteric pathogenicity S. vitro employing a polarized human colonic epithelial cell (T84) monolayer. Infected monolayers were assayed for bacterial invasion, transepithelial electrical resistance (TEER), cytotoxicity, interleukin-8 (IL-8) release morphological changes by scanning electron microscopy. observed significantly greater invasion compared to Escherichia coli strain HS (p = 0.0038 respectively). Cell was accompanied reduction TEER secretion IL-8. Lactate dehydrogenase (LDH) extracellular concentration rapidly increased within few hours exposure monolayer marcescens. Scanning microscopy marcescens-infected demonstrated destruction microvilli vacuolization. Our results suggest interacts intestinal cells culture induces dramatic alterations similar those produced known pathogens.