Effects of L-carnitine and its acetyl and propionyl esters on ATP and PCr levels of isolated rat hearts perfused without fatty acids and investigated by means of 31P-NMR spectroscopy.

摘要: 31P-NMR in vivo spectroscopy is a non-invasive and non-hazardous technique which investigates chemical composition metabolism of living objects, for example by determining phosphocreatine (PCr) ATP concentrations. In the present study we investigated influence L-carnitine, acetyl-L-carnitine propionyl-L-carnitine on energetic state Langendorff rat heart subjected to an ischemic period 20 min followed reperfusion 60 min. To avoid overlapping effects fatty acids glucose, hearts were perfused with Tyrode solution containing no acids. Ischemia causes rapid decrease PCr signal, signal after prolonged ischemia. At same time, drastic increase Pi was observed. A partial recovery signals observed period. With L-carnitine markedly improved high energy phosphates (e.g. increased PCr/Pi ratios) found. this effect enhanced first postischemic phase. It followed, however, more ratio late The not significantly minutes period, but during whole phase stabilization Intracellular pH can be calculated from determination Pi-chemical shift. This shows that its derivatives have protective against intracellular improves heart, leads ischemia tolerance. Hearts under able tolerate up four ischemia-reperfusion periods succession, whereas controls do so. These NMR results confirm hypothesis esters heart. could importance treatment cardiac diseases.