Alkaline peroxide pretreatment of corn stover: effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose

摘要: Pretreatment is a critical step in the conversion of lignocellulose to fermentable sugars. Although many pretreatment processes are currently under investigation, none them entirely satisfactory regard effectiveness, cost, or environmental impact. The use hydrogen peroxide at pH 11.5 (alkaline (AHP)) was shown by Gould and coworkers be an effective grass stovers other plant materials context animal nutrition ethanol production. Our earlier experiments indicated that AHP performed well when compared against two alkaline pretreatments. Here, we explored several key parameters test potential for further improvement relevant lignocellulosic effects biomass loading, residence time, control were tested combination with subsequent digestion commercial enzyme preparation, optimized mixtures four enzymes, synthetic pure enzymes. room temperature (23°C) atmospheric pressure, after neutralized HCl but not washed before digestion. Standard conditions 0.2% glucan 15 mg protein/g glucan, 48 h 50°C. Higher loadings (10% 20%) gave higher monomeric glucose (Glc) xylose (Xyl) yields than 2% loading used studies. An H2O2 0.25 g/g almost as 0.5 g/g, 0.125 significantly less effective. Optimized enzymes substantially increased post-AHP-pretreatment enzymatic hydrolysis all concentrations any single enzyme. At 10% biomass, mixture total protein 8 mg/g Glc 83% 95%, respectively. Yields Xyl low (0.125 g H2O2/g biomass) could improved extending time readjusting every 6 during pretreatment. A yield 77% obtained using 15% adjustment, followed glucan. Alkaline corn stover. Particular advantages reagents impact avoidance special reaction chambers. Reasonable can concentration one-quarter previous research. Additional improvements process, such stabilization, recycling, control, lead