Voltage-gated sodium channels potentiate the invasive capacities of human non-small-cell lung cancer cell lines.

摘要: Ionic channel activity is involved in fundamental cellular behaviour and participates cancerous features such as proliferation, migration invasion which turn contribute to the metastatic process. In this study, we investigated expression role of voltage-gated sodium channels non-small-cell lung cancer cell lines. Functional was normal The measurement, patch-clamp conditions, tetrodotoxin-inhibitable currents indicated that strongly lines H23, H460 Calu-1 possess functional while weakly do not. While all expressed mRNA for numerous isoforms, only cells had a 250 kDa protein corresponding channel. other also another 230 not addressed membrane might act dominant negative isoform prevent activation. At potential these cells, are partially open. This leads continuous entry sodium, disrupting homeostasis down-stream signaling pathways. Inhibition by tetrodotoxin responsible 40-50% reduction vitro invasion. These experiments suggest be an integral component process probably through its involvement regulation intracellular homeostasis. could serve both novel markers phenotype new therapeutic targets.